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Updated: Jan 29, 2026

A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells
Published on: April 13, 2021
Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells
Yasuhiro Yoshimura1, Atsuhiro Taguchi2, Ryuichi Nishinakamura3
1Department of Kidney Development, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.
Abstract:
Nephrons, the functional units of the kidney, are derived from nephron progenitor cells (NPCs). Here, we describe methods to reconstruct nephron tissue via induction of NPCs from mouse and human pluripotent stem cells, which mimic multistep developmental signals in vivo. Induced NPCs differentiate into three-dimensional nephron structures, including glomerular podocytes and nephric tubules, which are useful for studying early stages of kidney specification and morphogenetic processes in the context of normal development or disease.
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