Transformation and regeneration of garlic (Allium sativum L.) by Agrobacterium-mediated gene transfer
T Kondo1, H Hasegawa1, M Suzuki1
1Aomori Green BioCenter, 221-10, Yamaguchi, Nogi, Aomori, 030-0142, Japan e-mail: msuzuki@mm.neweb.ne.jp Fax: +81-177-281017, , , , , , JP.
Abstract:
By using highly regenerative calluses, we developed a stable transformation system in garlic (Allium sativum L.). The temperature and number of days of co-cultivation with Agrobacterium tumefaciens was shown to be an important factor in transient expression of the uid A gene. After a culture period of 5 months in selection medium containing hygromycin, 20 shoots were induced from ca. 1000 calluses, among which 15 plants expressed β-glucuronidase activity upon staining with X-Gluc. Shoots developed into transgenic garlic after 1 month. Integration of the uid A gene was confirmed by Southern blot analysis for genomic DNA of transgenic garlic plants.
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