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Amyloid Fibrils03:03

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Related Experiment Video

Updated: Jan 29, 2026

Assaying β-amyloid Toxicity using a Transgenic C. elegans Model
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Small Molecule Amyloid-β Protein Precursor Processing Modulators Lower Amyloid-β Peptide Levels via cKit Signaling.

Ci-Di Chen1, Ella Zeldich1, Christina Khodr1

  • 1Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA.

Journal of Alzheimer'S Disease : JAD
|February 19, 2019
PubMed
Summary

Compound Y10 reduces amyloid-beta (Aβ) levels in Alzheimer's disease (AD) models by targeting the cKit receptor tyrosine kinase. This inhibition impacts AβPP phosphorylation and Aβ production, suggesting a novel therapeutic approach.

Keywords:
High throughput screeningkinaseneurodegenerationphosphatasephosphorylation

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Pharmacology

Background:

  • Alzheimer's disease (AD) involves amyloid-beta (Aβ) peptide and tau protein accumulation.
  • Inhibiting Aβ production is a potential therapeutic strategy for AD.
  • Previous screens identified a compound reducing Aβ levels via AβPP dimerization inhibition.

Purpose of the Study:

  • To investigate compound Y10, an analog that reduces Aβ levels.
  • To elucidate the mechanism of Y10 action, focusing on AβPP phosphorylation and Aβ production.
  • To explore the role of the cKit signaling pathway in AβPP regulation.

Main Methods:

  • High-throughput screening for AβPP dimerization inhibitors.
  • Kinase profiling to identify Y10 target.
  • Immunoprecipitation-western blotting to examine AβPP phosphorylation.
  • Use of cKit and Shp2 inhibitors and siRNA.
  • Assessment of AβPP surface localization.

Main Results:

  • Compound Y10 reduced Aβ levels and inhibited cKit phosphorylation.
  • Y10 increased AβPP phosphorylation at tyrosine Y743.
  • Inhibition of cKit and Shp2 phosphatase increased AβPP phosphorylation and reduced Aβ levels.
  • Inhibitors of cKit and Shp2 enhanced AβPP surface localization.

Conclusions:

  • The cKit signaling pathway regulates AβPP phosphorylation and Aβ production.
  • Targeting cKit and Shp2 offers a novel therapeutic strategy for AD.
  • Regulation of AβPP phosphorylation by small molecules is a promising intervention for Alzheimer's disease.