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Updated: Jan 29, 2026

Quantifying Agonist Activity at G Protein-coupled Receptors
Published on: December 26, 2011
GLP-1 receptor agonist impairs keratinocytes inflammatory signals by activating AMPK
Jing Yang1, Zongming Wang1, Xilin Zhang1
1Department of Dermatology and Venerology,Chongqing Three Gorges Central Hospital, ChongQing 404100, China.
Purpose:
Liraglutide, a glucagon-like peptide-1 (GLP-1) analogue, is an antidiabetic drug. It has been shown to improve the Psoriasis Area and Severity Index (PASI) in patients with type 2 diabetes and psoriasis in clinical practice, but the mechanism remains somewhat unclear. We used lipopolysaccharides (LPS) to induce inflammatory response in keratinocytes and explore the mechanism.
Methods:
The HaCat cells were incubated with LPS for 16 h and then were treated with liraglutide for 30 min. Cell viability was testing by CCK-8 assay. GLP-1Rs and intracellular signaling pathways were identified by Western blot. The migration of macrophage was detecting by trans-well assay.
Results:
Liraglutide decreased cell viability in the HaCat cells. Liraglutide restrained the migration of macrophage to the HaCat cells. LPS elevated not only the protein abundance of phospho-IKKα/β S176/S180, phospho-NF-κB p65, phospho-JAK2, phospho-STAT3 and SOCS3, but also the levels of TNF-α and IL-6 in the HaCat cells. These effects of LPS were reversed by liraglutide. In addition, liraglutide increased phosphorylation of AMPK. The AMPK inhibitor Compound (CC) impaired liraglutide-inhibited p-NF-κB p65 and p-STAT3.
Conclusions:
GLP-1 impaired keratinocytes inflammatory signals by activating AMPK and restrained macrophage migration.
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