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Updated: Jan 29, 2026
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Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs
Published on: March 18, 2016
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SILAC-based quantitative proteomics identifies size-dependent molecular mechanisms involved in silver
M N Fernández1, R Muñoz-Olivas1, J L Luque-Garcia1
1a Faculty of Chemical Sciences, Department of Analytical Chemistry , Complutense University of Madrid , Madrid , Spain.
Nanotoxicology
|February 20, 2019
Summary
Silver nanoparticles (NPs) exhibit size-dependent toxicity. Smaller 10nm silver NPs cause nucleolar stress, while both 10nm and 60nm silver NPs induce DNA damage and oxidative stress, impacting cell proliferation and survival.
Area of Science:
- Nanotechnology
- Toxicology
- Biochemistry
Background:
- Silver nanoparticles (NPs) are widely used due to their antibacterial properties.
- Increasing human and environmental exposure necessitates understanding silver NP toxicity.
- The size-dependent effects of silver NPs on biological systems are not fully understood.
Purpose of the Study:
- To investigate the size-dependent biomolecular mechanisms of silver NP toxicity.
- To compare the toxicological effects of 10nm and 60nm silver NPs on hepatic cells.
Main Methods:
- Quantitative proteomic analysis was performed on hepatic cells.
- Cells were exposed to 10nm and 60nm silver nanoparticles.
- Differential protein expression was analyzed to identify affected cellular pathways.
Main Results:
- 10nm silver NPs induced nucleolar stress and halted ribosome biogenesis.
- Both 10nm and 60nm silver NPs caused DNA damage and oxidative stress via distinct pathways.
- Cell proliferation, cell cycle, and apoptosis were negatively impacted by both NP sizes.
Conclusions:
- Silver NP toxicity is size-dependent, affecting different biomolecular pathways.
- Understanding these size-specific mechanisms is crucial for risk assessment and application development.
- Silver NPs can induce genotoxicity, oxidative stress, and affect cell cycle regulation.
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