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A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity
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Cytotoxic Activity of Peripheral Blood NK Cells towards Trophoblast Cells during Pregnancy

V A Mikhailova1, D O Bazhenov2, L P Viazmina2

  • 1D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology, St. Petersburg, Russia. mva_spb@mail.ru.

Bulletin of Experimental Biology and Medicine
|February 22, 2019
PubMed

We evaluated cytotoxic activity of peripheral blood NK cells towards trophoblast cells. NK cells either isolated or in the composition of mononuclear cell fraction, caused death of trophoblast cells. In women with physiological pregnancy, the cytotoxic effect of NK cells present in mononuclear cell fraction preincubated with IL-2 was lower than in nonpregnant women, who had never been pregnant previously, and in fertile women. Cytotoxic activity of isolated NK cells preincubated with IL-2 in fertile women was lower than in nonpregnant women, who had never been pregnant previously.

Keywords:
JEG-3NK cellscytotoxic activityproliferationtrophoblast

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