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Identification of Circulating Endothelial Colony-Forming Cells from Murine Embryonic Peripheral Blood

Yang Lin1,2, Chang-Hyun Gil1, Mervin C Yoder3,4

  • 1Department of Pediatrics, Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2019
PubMed

Human umbilical cord blood contains highly proliferative circulating endothelial colony-forming cells (ECFC). These cells have promising therapeutic potential for various cardiovascular diseases by possessing robust in vitro clonal expansion potential and the ability to form functional blood vessels in vivo upon transplantation into recipient immunodeficient mice. However whether similar cells also exist in murine blood remains unresolved, which impedes the study of circulating ECFC biology using murine models. Here we describe a method to identify and culture murine embryonic peripheral blood-derived circulating ECFC through co-culture with OP9 stromal cells. Using this method, embryonic circulating ECFC can be identified by the formation of sheet-like or network-like endothelial colonies upon OP9 stromal cell monolayers.

Keywords:
Circulating endothelial cellsEndothelial colony-forming cellsEndothelial progenitor cellsMouse embryoOP9 stromal cellsPeripheral blood

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