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Development of a sensitive enzyme-linked immunosorbent assay for the detection of diacetoxyscirpenol
U Klaffer1, E Märtlbauer, G Terplan
1Lehrstuhl für Hygiene und Technologie der Milch, München, F.R.G.
Abstract:
An enzyme-linked immunosorbent assay (ELISA) for the detection of diacetoxyscirpenol (DAS) was developed. Antibodies against DAS were obtained after immunization of rabbits with DAS-hemiglutarate-human serum albumin (DAS-HG-HSA). After an immunization period of 6 weeks a sufficient level of specific antibodies was reached. A DAS-hemisuccinate-horseradish peroxidase-conjugate (DAS-HS-HRP) was prepared by an ester method and used as enzyme-labelled toxin in the competitive assay. The detection limit for DAS was about 10 pg/ml. The relative cross reactivity of the assay was 597.5, 5.2, 100.0, 2.5, and 1.5% for 3 alpha-acetyl-DAS, DAS, T-2 toxin, neosolaniol, and 15-acetoxyscirpenol, respectively.