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Updated: Jan 28, 2026

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
Methods for Detecting N-Acyl Homoserine Lactone Production in Acinetobacter baumannii
Aimee R Paulk Tierney1, Philip N Rather2
1Department of Microbiology and Immunology, Emory University School of Medicine, Emory University, Atlanta, GA, USA.
Abstract:
Acinetobacter baumannii cell-cell signaling is mediated by production and release of N-acyl homoserine lactones (AHLs), primarily N-(3-hydroxydodecanoyl)-L-homoserine lactone (3-hydroxy-C12-HSL). The secretion of this signal can be readily assessed via simple plate-based or thin-layer chromatography-based assays utilizing an Agrobacterium tumefaciens traG-lacZ containing biosensor. In this chapter, we describe methods to assay for secreted N-acyl homoserine lactone production in Acinetobacter baumannii.
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