Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Synthesis and Decomposition Reactions

Synthesis and Decomposition Reactions

Synthesis and decomposition are two types of redox reactions. Synthesis means to make something, whereas decomposition means to break something. The reactions are accompanied by chemical and energy changes.

Flow Sheet

Flow Sheet

Flowsheets are valuable tools in nursing documentation. They enable healthcare professionals to efficiently record and monitor various patient assessments and measurements in a consolidated format.
Here's a closer look at the examples of flowsheets commonly used by nurses:
Graphic Sheet Documentation:

Dehydration Synthesis

Dehydration Synthesis

Overview
Dehydration synthesis (also called a condensation reaction) is the chemical process in which two molecules covalently link together to form a new molecule, along with the release of a water molecule. Many physiologically important compounds form by dehydration synthesis reactions, such as complex carbohydrates, proteins, DNA, and RNA.
Synthesis of carbohydrates
Sugar molecules are covalently linked together by dehydration synthesis. During the reaction, the hydroxyl (-OH) group from...

Lagging Strand Synthesis

Lagging Strand Synthesis

During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...

Light Acquisition

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.

The Wave Nature of Light

The Wave Nature of Light

The nature of light has been a subject of inquiry since antiquity. In the seventeenth century, Isaac Newton performed experiments with lenses and prisms and was able to demonstrate that white light consists of the individual colors of the rainbow combined together. Newton explained his optics findings in terms of a "corpuscular" view of light, in which light was composed of streams of extremely tiny particles traveling at high speeds according to Newton's laws of motion.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Modulation of Oncogenic KRAS Signaling by Branched Actin-driven Cell Membrane Protrusions.

Research square·2026

Same author

Altair-dvOPM: an open-access platform for large-field three-dimensional tissue imaging.

bioRxiv : the preprint server for biology·2026

Same author

A quantitative pipeline for whole-mount deep imaging and analysis of multi-layered organoids across scales.

eLife·2026

Same author

Modulation of Oncogenic KRAS Signaling by Branched Actin-driven Cell Membrane Protrusions.

bioRxiv : the preprint server for biology·2026

Same author

An epithelial morphogenetic program for maximal urine concentration.

Nature communications·2026

Same author

Leptin Receptor <sup>+</sup> cells create a perisinusoidal niche for thrombopoiesis in the bone marrow by synthesizing CXCL14.

bioRxiv : the preprint server for biology·2026

Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026

Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026

Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026

Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026

Same journal

Deep molecular profiling in three dimensions.

Nature methods·2026

Same journal

3D pathology-guided microdissection.

Nature methods·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 28, 2026

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

Published on: September 28, 2022

Universal light-sheet generation with field synthesis.

Bo-Jui Chang¹, Mark Kittisopikul^2,3, Kevin M Dean¹

¹Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX, USA.

|February 27, 2019

Summary

This summary is machine-generated.

Field synthesis creates light sheets for microscopy using simple 1D intensity patterns. This method simplifies optical design, improves light efficiency, and reduces photobleaching compared to lattice light-sheet microscopy.

More Related Videos

Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function

Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function

Published on: January 5, 2024

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Published on: September 15, 2018

Related Experiment Videos

Last Updated: Jan 28, 2026

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

Published on: September 28, 2022

Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function

Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function

Published on: January 5, 2024

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Published on: September 15, 2018

Area of Science:

Optics and Photonics
Microscopy Techniques
Biophysics

Background:

Lattice light-sheet microscopy (LLSM) is a powerful technique for biological imaging.
Existing methods for generating light sheets can be complex and prone to photobleaching.

Purpose of the Study:

To introduce a novel theorem and method called field synthesis.
To provide a simpler, more efficient alternative for generating light sheets in microscopy.

Main Methods:

Field synthesis utilizes an incoherent superposition of one-dimensional intensity distributions.
This method can synthesize any scanned or dithered light sheet, including those used in LLSM.

Main Results:

Field synthesis offers a simplified optical design compared to LLSM.
The method achieves higher light throughput and enables simultaneous multicolor illumination.
It also results in lower rates of photobleaching than lateral beam scanning methods.

Conclusions:

Field synthesis is a user-friendly and modular approach for generating microscopy light sheets.
This technique presents significant advantages in terms of optical design, efficiency, and phototoxicity.