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Updated: Jan 28, 2026
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Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
Published on: September 28, 2016
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Determination of Cell-Surface Hyaluronan Through Flow Cytometry.
Daiana L Vitale1, Fiorella M Spinelli1, Laura Alaniz2
1Laboratorio de Microambiente Tumoral, Universidad Nacional del Noroeste de la Pcia. de Bs. As, Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires (CITNOBA UNNOBA-CONICET), Junín, Buenos Aires, Argentina.
Methods in Molecular Biology (Clifton, N.J.)
|March 3, 2019
Summary
Hyaluronan, a key extracellular matrix component, influences cell behavior. Its interactions with hyaladherins can be analyzed using flow cytometry for research insights.
Area of Science:
- Biochemistry
- Cell Biology
- Glycobiology
Background:
- Hyaluronan (HA) is a major glycosaminoglycan in the extracellular matrix.
- HA synthesis occurs at the cellular plasma membrane.
- Altered HA expression impacts cell proliferation, adhesion, and migration.
Purpose of the Study:
- To explore the role of hyaluronan in cellular processes.
- To investigate the interaction of hyaluronan with binding proteins (hyaladherins).
- To establish a method for analyzing these interactions.
Main Methods:
- Hyaluronan expression analysis.
- Identification and characterization of hyaluronan-binding proteins (hyaladherins).
- Conjugation of hyaladherins to fluorochromes.
- Flow cytometry analysis of hyaluronan-hyaladherin interactions.
Main Results:
- Hyaluronan plays a significant role in cell behavior.
- Hyaladherins bind to hyaluronan.
- Flow cytometry enables the analysis of these interactions.
Conclusions:
- Hyaluronan is crucial for cell function.
- Hyaladherin interactions with hyaluronan can be quantified.
- Flow cytometry is a viable method for studying hyaluronan-related cellular events.
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