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Updated: Jan 28, 2026

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
Published on: February 19, 2020
Component Profiling in Agricultural Applications Using an Adjustable Acupuncture Needle for Sheath-Flow Probe
Kenzo Hiraoka1, Stephanie Rankin-Turner1,2, Satoshi Ninomiya3
1Clean Energy Research Center , University of Yamanashi , 4-3-11, Takeda , Kofu , Yamanashi 400-8511 , Japan.
Abstract:
In previous work, probe electrospray ionization/mass spectrometry (PESI/MS) and sheath-flow probe electrospray ionization/mass spectrometry (sfPESI/MS) were reported for the rapid and minimally invasive analysis of food. In this work, a modified version of sfPESI will be reported. The sample surface was pricked with an acupuncture needle inserted in the sfPESI probe that protruded from the terminus of the tip by 5 mm. The invasion depth of the needle into the sample was ∼1 mm. After sampling, the needle was retracted into the solvent-preloaded capillary with a protrusion length of 0.1-0.2 mm from the tip. A mass spectrum of the sample captured on the needle was obtained by applying a high voltage to the needle. This method could be applicable to profiling analyses of plants with the epicuticular wax covering on the surfaces that are difficult to analyze by sf-PESI. The on-site mass spectrometric analysis for a growing apricot in the field was performed to monitor the developing stage of the fruit.
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