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A Promising Probiotic Irrigant: An In Vitro Study
Hoda El-Sayed1, Yousra Aly2, Hanaa Elgamily2
1National Research Centre, Dairy Science Department (Microbiology Lab.), Food Industries and Nutrition Division, Giza, Egypt.
Open Access Macedonian Journal of Medical Sciences
|March 6, 2019
Summary
Lactobacillus rhamnosus demonstrated an inhibitory effect against Enterococcus faecalis in root canals. This probiotic irrigant shows potential as a safe, natural alternative for dental applications.
Area of Science:
- Endodontics
- Microbiology
- Probiotics
Background:
- Enterococcus faecalis is a common pathogen in persistent root canal infections.
- Conventional irrigants like sodium hypochlorite (NaOCl) have limitations and potential side effects.
- Exploring natural and safe alternatives for root canal irrigation is crucial.
Purpose of the Study:
- To evaluate the inhibitory efficacy of Lactobacillus rhamnosus (B-445) as a probiotic irrigant against Enterococcus faecalis.
- To assess the survival of Lactobacillus rhamnosus in a root canal environment infected with Enterococcus faecalis.
Main Methods:
- Human anterior teeth were instrumented and inoculated with Enterococcus faecalis.
- Teeth were irrigated with 2.5% NaOCl (positive control), saline (negative control), or Lactobacillus rhamnosus probiotic.
- Colony-forming units (CFUs) of Enterococcus faecalis and Lactobacillus rhamnosus were quantified at different time points.
Main Results:
- Sodium hypochlorite exhibited the strongest inhibition of Enterococcus faecalis, followed by Lactobacillus rhamnosus.
- The probiotic irrigant group showed significantly lower Enterococcus faecalis counts compared to the saline group.
- Lactobacillus rhamnosus demonstrated a favorable survival profile within the infected root canal.
Conclusions:
- Lactobacillus rhamnosus exhibits significant inhibitory effects on Enterococcus faecalis growth.
- This probiotic agent presents a promising, natural, and safe alternative for root canal irrigation.
- Further research into probiotic irrigants could revolutionize endodontic treatment protocols.
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