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Layer-By-Layer Film Engineering for Sequential Gene Delivery.

Lingxiao Xie1, Yi Zou1, Sean Carroll1,2

  • 1Department of Chemical Engineering and Materials Science, Wayne State University, Detroit, MI, USA.

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|March 7, 2019
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Summary
This summary is machine-generated.

Layer-by-layer (LbL) films with bioreducible poly(amido amine)s (PAAs) and DNA plasmids degrade in physiologic conditions. Barrier layers stabilize these films for sequential gene delivery to HEK 293 cells.

Keywords:
Atomic force microscopyBioreducible poly(amido amine)DNA deliveryFilm degradationLayer-by-layer (LbL) filmPolyelectrolyte multilayersSequential gene delivery

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Gene Delivery

Background:

  • Layer-by-layer (LbL) assembly offers precise control over film architecture.
  • Bioreducible polymers are being explored for triggered degradation in physiological environments.
  • Controlled gene delivery systems are crucial for therapeutic applications.

Purpose of the Study:

  • To develop and characterize bioreducible LbL films for controlled gene delivery.
  • To investigate the role of barrier layers in film stability and degradation.
  • To demonstrate localized gene delivery using these advanced LbL films.

Main Methods:

  • Fabrication of LbL films using poly(amido amine)s (PAAs) and DNA plasmids.
  • Characterization of film interior structure using atomic force microscopy (AFM), ellipsometry, dynamic light scattering (DLS), and fluorescence spectroscopy.
  • Assessment of film degradation in the presence of glutathione and redox-active compounds.
  • Demonstration of gene delivery to human embryonic kidney 293 (HEK 293) cells.

Main Results:

  • LbL films incorporating PAAs exhibited degradation in physiologic conditions due to bioreducible disulfide bonds.
  • Barrier layers were found to be essential for stabilizing the film structure and modulating degradation rates.
  • Sequential gene delivery was achieved by controlling film degradation.
  • Localized gene delivery to HEK 293 cells was successfully demonstrated.

Conclusions:

  • Bioreducible LbL films offer a promising platform for controlled and localized gene delivery.
  • The incorporation of barrier layers is critical for achieving tunable degradation and sequential release.
  • This approach holds potential for advanced therapeutic gene delivery strategies.