Related Experiment Video

Updated: Jan 27, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
09:20

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

7.3K

Precise in vivo tissue micro-Raman spectroscopy with simultaneous reflectance confocal microscopy monitoring using a

Zhenguo Wu, Liwei Jiang, Wenbo Wang

    Optics Letters
    |March 16, 2019
    PubMed

    More Related Videos

    Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy10:59

    Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy

    Published on: May 12, 2023

    3.4K
    In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy09:06

    In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy

    Published on: December 20, 2021

    3.8K

    Related Concept Videos

    Raman Spectroscopy: Overview01:20

    Raman Spectroscopy: Overview

    The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
    However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
    1.5K
    Raman Spectroscopy Instrumentation: Overview01:26

    Raman Spectroscopy Instrumentation: Overview

    A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
    The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
    1.1K
    Confocal Fluorescence Microscopy01:16

    Confocal Fluorescence Microscopy

    Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
    21.0K
    Total Internal Reflection Fluorescence Microscopy01:05

    Total Internal Reflection Fluorescence Microscopy

    Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
    13.4K
    Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

    Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

    Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
    The ATR process begins by directing a beam...
    1.2K
    Reflection of Waves01:07

    Reflection of Waves

    When a wave travels from one medium to another, it gets reflected at the boundary of the second medium. A common example of this is when a person yells at a distance from a cliff and hears the echo of their voice. The sound waves (longitudinal waves) traveling in the air are reflected from the bounding cliff. Similarly, flipping one end of a string whose other end is tied to a wall causes a pulse (transverse wave) to travel through the string, which gets reflected upon reaching the wall. In...
    4.6K