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Updated: Jan 27, 2026

Assessment of Boron Doped Diamond Electrode Quality and Application to In Situ Modification of Local pH by Water Electrolysis
Published on: January 6, 2016
Captavidin as a regenerable biorecognition element on boron-doped diamond for biotin sensing
Alyah Buzid1, Phyllis E Hayes1, Jeremy D Glennon1
1Innovative Chromatography Group, Irish Separation Science Cluster (ISSC) Ireland, University College Cork, Western Road, Cork, T12 YN60, Ireland; School of Chemistry and Analytical & Biological Chemistry Research Facility (ABCRF), University College Cork, College Road, Cork, T12 YN60, Ireland.
Abstract:
Captavidin, a nitrated avidin with moderate affinity with biotin, irreversibly adsorbs on carboxymethylcellulose to form a regenerable biorecognition element for biotin. This layer was retained and stabilized on a boron-doped diamond electrode by a Nafion film for repeated impedance analyses of biotin down to below 1 nM. The labeless electrochemical sensing scheme was then demonstrated for the analysis of biotin in blood plasma. The incorporation of captavidin confers detection specificity and regenerability, whereas the Nafion and carboxymethylcellulose layers circumvent the diffusion of endogenous electroactive species. The biosensing layer is simply regenerated by applying oxidation of +2 V for 1 min instead of its submersion in carbonate buffer for 10 min.
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