Capillary electrophoretic apparatus for the endpoint detection in microtitration methods.

Area of Science:

• Analytical Chemistry
• Biochemistry
• Pharmaceutical Analysis

Background:

• Conventional titration methods (CTM) are widely used but require large sample volumes, limiting their application for precious or limited samples.
• Existing microtitration devices are not always available, posing challenges for small-scale analyses.
• Accurate quantification of acids, bases, metal ions, and drugs is crucial in various scientific and industrial fields.

Purpose of the Study:

• To demonstrate the utility of a commercial capillary electrophoretic apparatus (CEa) for microtitration endpoint detection.
• To develop a cost-effective and accessible microtitration method requiring minimal sample volumes.
• To explore the application of conductivity changes for precise endpoint determination in microtitration.

Main Methods:

• Utilized a commercial capillary electrophoretic apparatus (CEa) for real-time monitoring during titration.
• Exploited the principle of solution conductivity changes to detect the titration endpoint.
• Recorded electrical current variations against added titrant volume to generate titration curves.

Main Results:

• Successfully demonstrated the use of CEa for accurate endpoint detection in microtitration.
• Required only 1.1-1.5 mL of analyte solutions to establish reliable titration curves.
• Equivalent points were easily identified by plotting the change in electrical current versus titrant volume.

Conclusions:

• The proposed capillary electrophoresis-assisted microtitration is a quick, easy, and efficient method for quantitative analysis.
• This technique significantly reduces sample consumption, making it suitable for expensive and limited samples.
• The method offers a practical alternative to conventional titration methods, especially in resource-constrained settings.