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Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery
Published on: November 5, 2019
Meningococcal Serogroup A, B, C, W, X, and Y Serum Bactericidal Antibody Assays
Jay Lucidarme1, Jennifer Louth2, Kelly Townsend-Payne2
1Meningococcal Reference Unit, National Infection Service, Public Health England, Manchester Medical Microbiology Partnership, Manchester Royal Infirmary, Manchester, UK.
Abstract:
Serum bactericidal antibody (SBA) assays measure functional antibody titers against Neisseria meningitidis in sera. Induction of complement-dependent SBA after vaccination with meningococcal polysaccharide or conjugate or protein based vaccines is regarded as the surrogate of protection and thus acceptable evidence of the potential efficacy of these vaccines. This chapter discusses and details SBA assay protocols for measuring the complement-mediated lysis of serogroup A, B, C, W, X, and Y meningococci by human sera, for example, following vaccination or disease.
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