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Updated: Jan 27, 2026

Protocol for Culturing Sympathetic Neurons from Rat Superior Cervical Ganglia SCG
Published on: January 30, 2009
[Age-development changes of the sympathetic innervation of the rat stomach.]
A I Emanuilov1, V V Konovalov1, P M Masliukov1
1Yaroslavl State Medical University, 5 Revolucionnaya str., Yaroslavl 150000, Russian Federation;
Abstract:
Sympathetic innervation of the stomach is carried out by the prevertebral ganglia of the solar plexus. The localization and neurochemical composition of neurons innervating the stomach in postnatal ontogenesis in rats was studied using the method of retrograde axon transport of Fast Blue. In all animals, the celiac ganglia had more labeled neurons compared to the superior mesenteric ganglion. The number of labeled neurons increased in the first 10 days of life and then did not change until the senescence. All labeled neurons innervating the stomach contain the catecholamine synthesis enzyme, tyrosine hydroxylase. The proportion of labeled neuropeptide Y-immunopositive neurons did not change in the development, the percentage of labeled calbindin-immunoreactive neurons decreased in the first month of life.
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