Related Experiment Video
Updated: Jan 27, 2026
06:51
Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures
Published on: December 13, 2019
6.6K
CIRP regulates BEV-induced cell migration in gliomas
Yu-Xiao Liu1, Jun-Nian Zhou2,3,4, Ke-Hui Liu5
1Department of Neurosurgery, The Fourth Medical Centre of Chinese PLA General Hospital, Beijing 100048, China, zhangzw301@163.com.
Cancer Management and Research
|March 19, 2019
Summary
Cold-inducible RNA-binding protein (CIRP) expression decreases with bevacizumab (BEV) treatment in glioma cells. Restoring CIRP inhibits migration by binding CXCL12 mRNA, suggesting a role in overcoming therapeutic resistance.
Area of Science:
- Oncology
- Molecular Biology
- Proteomics
Background:
- Malignant gliomas are aggressive brain tumors.
- Bevacizumab (BEV) is used to treat malignant glioma, but therapeutic resistance is common.
- Understanding molecular mechanisms of BEV resistance is crucial for improving treatment outcomes.
Purpose of the Study:
- To identify molecular signatures associated with bevacizumab (BEV) treatment failure in malignant glioma.
- To investigate the role of cold-inducible RNA-binding protein (CIRP) in glioma drug resistance and BEV treatment.
Main Methods:
- Quantitative proteomic analysis (2D LC-MS/MS, 6-plex iTRAQ) to identify protein expression changes after BEV treatment.
- Functional assays (invasion, xenograft) to assess CIRP's role in drug resistance.
- RNA-binding protein immunoprecipitation and microarray analysis to identify CIRP-bound mRNAs.
Main Results:
- Eighty-seven proteins showed significant fold changes post-BEV treatment, primarily involved in signal transduction, cell adhesion, and protein transport.
- CIRP expression decreased significantly after BEV treatment.
- Ectopic CIRP expression inhibited cell migration in BEV-treated glioma cells and reduced CXCL12 mRNA levels.
Conclusions:
- CIRP plays a role in bevacizumab (BEV)-induced migration of gliomas.
- CIRP may exert its function by binding migration-related RNAs, such as CXCL12.
- Targeting CIRP could be a potential strategy to overcome therapeutic resistance in glioma.
Related Concept Videos
01:19Cell Migration
6.5K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.5K
01:09Cell Migration
18.7K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.7K
01:28pH Regulation in Cells
7.6K
pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
7.6K
02:14GTPases and their Regulation
9.8K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒ small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
Large G-proteins,...
9.8K
02:23Master Transcription Regulators
7.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.8K
01:35Cancer Cell Migration through Invadopodia
3.3K
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.3K