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Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis
Published on: January 30, 2018
miR-338-3p regulates osteoclastogenesis via targeting IKKβ gene
Dequn Niu1, Zheng Gong2, Xuemin Sun3
1Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Bengbu Medical College, Bengbu, 233000, People's Republic of China.
Abstract:
This study determined the effects of miR-338-3p on osteoclast (OC) differentiation and activation. The change levels of miR-338-3p in differentiated OCs were investigated by microRNA microarray assay and quantitative real-time PCR analysis. The effects of miR-338-3p on the differentiation and activation of OCs were determined by tartrate-resistant acid phosphatase staining resorption activity assay and Western blot. Target genes of miR-338-3p were identified by target gene prediction and dual-luciferase reporter gene detection assay as well as Western blot. Results showed that miR-338-3p was markedly downregulated in differentiated OCs. miR-338-3p could inhibit the formation and absorption activity of OCs. Western blot showed that miR-338-3p could influence the change levels of OC differentiation-related proteins. Dual-luciferase reporter gene detection assay and Western blot both showed that miR-338-3p directly targeted IKKβ gene. In conclusion, miR-338-3p may affect the formation and activity of OCs by targeting the IKKβ gene.
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