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3,3'-thiodipropanol as a versatile refractive index-matching mounting medium for fluorescence microscopy
Milvia Iris Alata Tejedo1, Juan Carlos Martínez Cervantes1, Adrian Saul Jimenez Roldán2
1Centro de Investigaciones en Óptica, Loma Del Bosque 115, León C.P. 37150, Mexico.
Biomedical Optics Express
|March 21, 2019
Summary
3,3'-Thiodipropanol (TDP) is a novel mounting medium for fluorescence microscopy. It offers tunable refractive index and preserves dye fluorescence, enhancing biological sample imaging.
Area of Science:
- Microscopy
- Biochemistry
- Materials Science
Background:
- High-resolution fluorescence microscopy demands optimized sample preparation.
- Mounting media significantly influence image formation through optical and chemical properties.
Purpose of the Study:
- To evaluate 3,3'-thiodipropanol (TDP) as a novel mounting medium for fluorescence microscopy.
- To characterize TDP's performance in preserving fluorescence and its refractive index properties.
Main Methods:
- Tested TDP with filamentous actin labeled by fluorescent phalloidins.
- Experimentally determined TDP's refractive index dispersion curve.
- Measured the effect of TDP on the quantum yield of common fluorescent dyes.
Main Results:
- TDP demonstrated good performance as a mounting medium.
- The refractive index dispersion of pH-adjusted TDP was characterized and compared to commercial media.
- TDP exhibited compatibility with fluorescent actin staining and preserved dye fluorescence.
Conclusions:
- 3,3'-Thiodipropanol (TDP) is a promising mounting medium for fluorescence microscopy.
- Its tunable refractive index and compatibility with fluorescent dyes offer advantages for biological imaging.
- TDP can be mixed with water and 2,2'-thiodiethanol (TDE) to fine-tune optical properties.