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Elemental concentration gradients between subcellular compartments.

I L Cameron, J T Hansen, K E Hunter

    Journal of Cell Science
    |March 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

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    This study reveals how Euglena gracilis maintains elemental gradients using ultracentrifugation. Key findings show ion redistribution, particularly chloride, linked to cellular structures and chromatin condensation.

    Area of Science:

    • Cell Biology
    • Biophysics
    • Biochemistry

    Background:

    • Subcellular elemental gradients are crucial for cell function.
    • Euglena gracilis is a model organism for studying these gradients.
    • Understanding ion distribution mechanisms is essential for cell physiology.

    Purpose of the Study:

    • To investigate the mechanisms of subcellular elemental (Na, K, Cl) gradient establishment and maintenance in Euglena gracilis.
    • To analyze the impact of ultracentrifugal stratification on ion distribution within the cell.
    • To correlate ion concentrations with specific subcellular compartments and cellular events.

    Main Methods:

    • Ultracentrifugal stratification of Euglena gracilis intracellular contents without cell lysis.
    • Cryofixation of stratified and non-stratified cells.

    Related Experiment Videos

  • Energy-dispersive X-ray microanalysis of elemental concentrations in cryosections.
  • Comparison of elemental concentrations in chloroplast, nucleus, paramylon granules, and open cytoplasm.

    • Main Results:

    • Significant elemental concentration differences were observed between subcellular compartments in non-stratified cells.
    • Ultracentrifugation induced redistribution of ions, notably K and Cl, into the nucleus, correlating with chromatin condensation.
    • Chloride levels decreased in ribosome-depleted cytoplasm, suggesting adsorption to or entrapment in ultracentrifugable material.

    Conclusions:

    • Ultracentrifugal stratification is a viable method for studying subcellular elemental gradients in Euglena gracilis.
    • The maintenance of cytoplasmic chloride concentration is closely associated with ultracentrifugable cellular components.
    • Ion localization and redistribution are dynamic processes influenced by cellular structures and physiological states.