Related Experiment Video
Updated: Jan 27, 2026

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
Published on: November 28, 2015
A Method to Isolate CD34+ Mononuclear Cells from Canine Peripheral Blood.
Vanmathy Kasimanickam1, Ramanathan Kasimanickam1
1Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, Washington.
Researchers isolated endothelial progenitor cells (EPCs) from beagle dog blood. These cells show potential for neovascularization in treating ischemic injuries.
Area of Science:
- Stem cell biology
- Regenerative medicine
- Vascular biology
Background:
- Adult stem cells, including those in peripheral blood, can differentiate and transdifferentiate into various cell types.
- Identifying specific subsets capable of differentiating into mature endothelial cells is crucial for therapeutic applications.
- Endothelial progenitor cells (EPCs) play a key role in neovascularization.
Purpose of the Study:
- To describe a method for isolating and characterizing endothelial progenitor cells (EPCs) from adult beagle dog peripheral blood.
- To evaluate the expression of key genes (CD34, KDR, CD133) in isolated CD34+ cell fractions.
Main Methods:
- Peripheral blood collection from adult beagle dogs.
- Isolation of peripheral blood mononuclear cells (PBMNCs).
- Separation of CD34+ cells using immunomagnetic principles.
- Characterization via flow cytometry and gene expression analysis.
Main Results:
- Successful isolation and characterization of CD34+ cells from peripheral blood.
- Confirmation of CD34, KDR, and CD133 gene expression in the isolated fractions.
- Demonstration of a viable method for obtaining potential EPCs.
Conclusions:
- Efficient isolation methods for EPCs are essential for their ex vivo expansion and transplantation.
- This technique facilitates the study and potential therapeutic use of EPCs in treating ischemic injuries.
- The described protocol supports advancements in regenerative medicine for neovascularization.
More Related Videos
10:48Isolation and Cryopreservation of Highly Viable Human Peripheral Blood Mononuclear Cells From Whole Blood: A Guide for Beginners
Published on: October 25, 2024
09:45Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
Published on: January 1, 2017
Related Concept Videos
Assessment of blood pressure in brachial artery(one-step method)
Prepare for the Procedure:
Assessment of blood pressure in brachial artery(two-step method)
Protein Buffers in Blood Plasma and Cells
Certain amino acids can exist in a zwitterion state at a...
Overview Of Cell Separation And Isolation
Peripheral Nervous System: Ganglia and Nerves
Nerves
The nerve is a bundle of axons that serves as the communication highway in the PNS. Each nerve is ensheathed in a protective layer of connective tissue called the epineurium. This outermost layer safeguards the nerve and supports the...
Peripheral Artery Disease I: Introduction