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Heat Inactivation of Bile Salt-Stimulated Lipase Activity in Human Milk and Colostrum 1
S H L Pan1, C W Dill1, E S Alford1
1Department of Animal Science, Texas A&M University, College Station, Texas 77843 and Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030.
Abstract:
Time-temperature relationships for heat-inactivation of bile salt-stimulated lipase activity in human milk and colostrum were systematically measured using a pH-stat assay procedure with triolein as substrate. The enzyme was not affected in either menstruum at 45°C for 40 min. The enzyme was destroyed almost instantaneously at 60°C, and was slightly more heat-sensitive in colostrum than in milk. The bile salt-stimulated lipase(s) in human milk was more heat sensitive than lipase in bovine milk.
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