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Golgi Oncoprotein GOLPH3 Gene Expression Is Regulated by Functional E2F and CREB/ATF Promoter Elements
Beatriz Peñalver-González1, Jon Vallejo-Rodríguez2, Gartze Mentxaka3
1Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country UPV/EHU, 48080 Bilbao, Spain. beatriz.penalver@ehu.eus.
Genes
|April 3, 2019
Summary
Golgi biogenesis is linked to cell cycle progression. E2F transcription factors regulate Golgi genes like GOLPH3, impacting the G1 to S phase transition and ensuring proper cell division.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Golgi duplication ensures even organelle segregation between daughter cells post-mitosis.
- Regulation of Golgi biogenesis during interphase remains poorly understood.
Purpose of the Study:
- Investigate the link between cell cycle regulation and Golgi growth during interphase.
- Examine the role of E2F transcription factors in regulating Golgi protein gene expression.
Main Methods:
- Analyzed messenger RNA (mRNA) expression of Golgi genes (GOLPH3, GOLGA2).
- Assessed promoter activity using luciferase reporter constructs and mutated E2F motifs.
- Utilized small interfering RNA (siRNA) to deplete GOLPH3 expression.
Main Results:
- GOLPH3 and GOLGA2 mRNA expression is induced in G1 phase.
- E2F1-3 transcription factors induce GOLPH3 promoter activity, while E2F7 represses it.
- GOLPH3 depletion delays the G1 to S phase transition.
Conclusions:
- E2F-mediated regulation of Golgi genes is crucial for timely cell cycle progression.
- Establishes a connection between cell cycle control and Golgi organelle function.
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