Related Experiment Video
Updated: Jan 26, 2026

Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine
Published on: February 16, 2018
Expanding the genetic code with a lysine derivative bearing an enzymatically removable phenylacetyl group
Marie Reille-Seroussi1, Susanne V Mayer, Wolfgang Dörner
1Institute of Biochemistry, University of Muenster, Wilhelm-Klemm-Str. 2, 48149 Münster, Germany. Henning.Mootz@uni-muenster.de.
Abstract:
We report the genetically encoded incorporation of phenylacetyl protected lysine (PacK) into proteins in Escherichia coli. This unnatural side-chain modification can be enzymatically removed using either penicillin G acylase (PGA) or, surprisingly, the sirtuin SrtN from Bacillus subtilis. Our approach expands the toolbox to reversibly control protein structure and function under very mild and non-denaturing conditions, as demonstrated by triggering the activity of the nonribosomal peptide synthetase GrsA.
More Related Videos
Related Concept Videos
lncRNA - Long Non-coding RNAs
What is Genetic Engineering?
What is Population Genetics?
Genetics of Speciation
Bearing Stress
Due to the intricacy of these microforces, an average value, known as bearing stress, is often used by...
Azimuths and Bearings

