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A Bioinformatic Analysis of MicroRNAs' Role in Human Intervertebral Disc Degeneration
Xue-Qiang Wang1,2, Wen-Zhan Tu1,2, Jia-Bao Guo1
1Department of Sport Rehabilitation, Shanghai University of Sport, Shanghai, China; †Department of Rehabilitation Medicine, Shanghai Shangti Orthopaedic Hospital, Shanghai, China; ‡Department of Rehabilitation Medicine, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Abstract:
Objectives The aim of our study was to ascertain the underlying role of microRNAs (miRNAs) in human intervertebral disc degeneration (IDD). Design Bioinformatic analysis from multiple databases. Methods Studies of the association of miRNAs and IDD were identified in multiple electronic databases. All potential studies were assessed by the same inclusion and exclusion criteria. We recorded whether miRNA expression was commonly increased or suppressed in the intervertebral disc tissues and cells of IDD subjects. We used String to identify biological process and cellular component pathways of differentially expressed genes. Results We included fifty-seven articles from 1,277 records in this study. This report identified 40 different dysregulated miRNAs in 53 studies, including studies examining cell apoptosis (26 studies, 49.06%), cell proliferation (15 studies, 28.3%), extracellular matrix (ECM) degradation (10 studies, 18.86%), and inflammation (five studies, 9.43%) in IDD patients. Three upregulated miRNAs (miR-19b, miR-32, miR-130b) and three downregulated miRNAs (miR-31, miR-124a, miR-127-5p) were considered common miRNAs in IDD tissues. The top three biological process pathways for upregulated miRNAs were positive regulation of biological process, nervous system development, and negative regulation of biological process, and the top three biological process pathways for downregulated miRNAs were negative regulation of gene expression, intracellular signal transduction, and negative regulation of biological process. Conclusions This study revealed that miRNAs could be novel targets for preventing IDD and treating patients with IDD by regulating their target genes. These results provide valuable information for medical professionals, IDD patients, and health care policy makers.
Insights
MicroRNAs (miRNAs) play a key role in human intervertebral disc degeneration (IDD). Targeting these miRNAs offers a novel approach for IDD prevention and treatment.
Area of Science:
- Biomedical Science
- Genetics
- Molecular Biology
Background:
- Intervertebral disc degeneration (IDD) is a significant health concern.
- The role of microRNAs (miRNAs) in IDD pathogenesis is not fully understood.
Purpose of the Study:
- To investigate the role of miRNAs in human IDD.
- To identify specific miRNAs and their associated pathways in IDD.
Main Methods:
- Bioinformatic analysis of multiple databases.
- Systematic review and inclusion/exclusion criteria for relevant studies.
- Identification of dysregulated miRNAs and pathway analysis using String.
Main Results:
- 57 articles were included, identifying 40 dysregulated miRNAs in IDD.
- Commonly dysregulated miRNAs include upregulated miR-19b, miR-32, miR-130b and downregulated miR-31, miR-124a, miR-127-5p.
- Pathways affected include cell apoptosis, proliferation, extracellular matrix degradation, and inflammation.
Conclusions:
- miRNAs are crucial in IDD development and progression.
- miRNAs represent potential therapeutic targets for IDD.
- Findings offer valuable insights for clinicians, patients, and policymakers.