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3D Capillary-Driven Paper-Based Sequential Microfluidic Device for Electrochemical Sensing Applications.

Abdulhadee Yakoh, Sudkate Chaiyo, Weena Siangproh1

  • 1Department of Chemistry, Faculty of Science , Srinakharinwirot University , Sukhumvit 23 , Wattana , Bangkok 10110 , Thailand.

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Summary

This study introduces a novel paper-based device for sequential reagent delivery in microfluidic assays. The capillary-driven platform simplifies complex biological detection without external power, enabling diverse applications.

Keywords:
electrochemical sensorsfolding papermicrofluidicpaper-based devicessequential fluid delivery

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Area of Science:

  • Microfluidics
  • Analytical Chemistry
  • Biosensors

Background:

  • Sequential reagent delivery in microfluidic paper-based devices often requires complex manipulation.
  • Existing electrochemical paper-based sensors can be limited by user intervention and convective mass transport.
  • There is a need for simplified, power-independent platforms for complex bioassays.

Purpose of the Study:

  • To design and fabricate a sequential fluid delivery platform on a microfluidic paper-based device (µPAD).
  • To demonstrate the device's capability for electrochemical detection of biological species using both flow-through and stopped-flow configurations.
  • To showcase the device's potential for simplifying complex assays and enabling diverse applications.

Main Methods:

  • Development of a 3D capillary-driven device comprising an origami folding paper (oPAD) and a reagent-stored pad (rPAD).
  • Fabrication of two configurations: flow-through for ascorbic acid detection and stopped-flow for serotonin and α-fetoprotein detection.
  • Electrochemical detection methods including voltammetry and impedimetric immunosensing were employed.

Main Results:

  • The flow-through configuration enabled self-calibration and real sample analysis for ascorbic acid.
  • The stopped-flow configuration successfully excluded convective mass transport disturbances for serotonin detection.
  • An impedimetric label-free immunosensor for α-fetoprotein was demonstrated using the modified stopped-flow device.
  • The device requires minimal sample volume (1 μL) and eliminates multi-step reagent handling.

Conclusions:

  • The developed microfluidic paper-based device offers a simple, power-independent solution for sequential fluid delivery.
  • The platform effectively performs complex electrochemical assays, including quantitative analysis and label-free immunosensing.
  • This innovative device holds significant promise for diverse point-of-care diagnostic and analytical applications.