Related Experiment Video
Updated: Jan 16, 2026
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
Published on: September 10, 2014
Dual electrochemical DNA sensor utilizing Ag(I)-Stabilized DNA mismatches for simultaneous, label-free detection of
Piyathida Phimdee1, Sudkate Chaiyo2, Abdulhadee Yakoh2
1Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok 10330, Thailand.
Abstract:
The WHO declared Mpox a public health emergency of international concern due to the resurgence of Mpox clade I outbreaks. Following, the CDC reported a high prevalence of HIV co-infection during the global MPOX outbreak. In 2025, no commercially available multiplex PCR assays enable concurrent detection of mpox and HIV. To address these limitations, this study presents a novel dual electrochemical DNA (eDNA) biosensor for the simultaneous, label-free detection of Mpox and HIV nucleic acids using a mismatched-driven nucleic acid-metal ion nanocomplex (MDN) strategy. The sensor exploits C-C mismatches that selectively coordinate with Ag+ ions, enabling covalent target capture and sensitive signal generation. It demonstrates a dynamic range of 101-105 pM with picomolar-level LODs and successful detection in clinical saliva and urine samples, with LOD below 4 copies/mL. This multiplex platform offers a rapid, cost-effective, and robust diagnostic tool for co-infection screening in high-risk populations.
More Related Videos
07:24Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection
Published on: October 29, 2020
07:16Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
Published on: February 9, 2024
