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Updated: Jan 26, 2026

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist
Published on: April 25, 2018
Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy
Kim C Jonas1,2,3, Aylin C Hanyaloglu4
1Molecular and Clinical Sciences Research Institute, St George's University of London, London, UK. kjonas@sgul.ac.uk.
Abstract:
Super-resolution imaging has provided unprecedented insight in the molecular complexities of fundamental cell biological questions. For G protein-coupled receptors (GPCRs), its application to the study of receptor homomers and heteromers have unveiled the diversity of complexes these GPCRs can form at the plasma membrane at a structural and functional level. Here, we describe our methodological approach of photoactivated localization microscopy with photoactivatable dyes (PD-PALM) to visualize and quantify the spatial assembly of GPCR heteromers at the plasma membrane.
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