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Updated: Jan 26, 2026
09:29
Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds
Published on: August 16, 2014
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[Structural control and characterization of hierarchically structured fibrous scaffolds]
Qiwei Li1, Chaojing Li1, Fujun Wang2
1Key Laboratory of Textile Science & Technology, Ministry of Education, Textile College of Donghua University, Shanghai, 201620, P.R.China.
Summary
This study developed novel hierarchically structured fibrous scaffolds using electrospinning and solution-induced methods. These PCL-based scaffolds exhibit tunable properties and good hemocompatibility and cytocompatibility for tissue engineering applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Materials Science
Background:
- Developing advanced fibrous scaffolds is crucial for tissue engineering.
- Tuning scaffold morphology and physicochemical properties impacts biological performance.
- Poly (e-caprolactone) (PCL) and polyvinylpyrrolidone (PVP) are common biomaterials.
Purpose of the Study:
- To create hierarchically structured fibrous scaffolds with diverse morphologies.
- To investigate how scaffold morphology influences physicochemical properties.
- To evaluate the hemocompatibility and cytocompatibility of these novel scaffolds.
Main Methods:
- Electrospinning of PCL/PVP bicomponent fibers followed by PVP extraction to create porous surfaces.
- Solution incubation method to form shish-kebab (SK) structures on PCL fibers.
- Characterization using scanning electron microscopy, contact angle, and differential scanning calorimetry.
- Hemolysis, coagulation, and cell proliferation (CCK-8) assays for biocompatibility assessment.
Main Results:
- Successfully fabricated porous and SK structured PCL scaffolds with tunable surface morphology.
- Increased crystallinity and hydrophobicity observed in structured scaffolds compared to smooth PCL.
- All scaffolds demonstrated non-hemolytic properties suitable for blood contact.
- Structured scaffolds showed enhanced endothelial cell proliferation compared to control.
Conclusions:
- Electrospinning combined with solution-induced and phase separation methods effectively constructs multi-scale scaffolds.
- These hierarchically structured scaffolds offer tunable physicochemical properties.
- The scaffolds exhibit good hemocompatibility and cytocompatibility, indicating high potential for tissue engineering.
Keywords:
Hierarchically structured fibrous scaffoldcell proliferationhemocompatibilityporous surfaceshish-kebab structureMore Related Videos
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