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Updated: Jan 26, 2026

Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
Published on: July 16, 2019
α-Selective Lysine Ligation and Application in Chemical Synthesis of Interferon Gamma
Yuankun Dao1, Lin Han1, Hanxuan Wang1
1State Key Laboratory of Natural and Biomimetic Drugs, and Department of Chemical Biology , School of Pharmaceutical Sciences, Peking University , Beijing 100191 , China.
Abstract:
A traceless β-mercaptan-assisted α-selective ligation of N-terminal lysine-containing peptides has been developed. In this ligation-desulfurization-based protocol, the ε-amine of lysine is free of protection, thus improving the overall synthetic efficiency and avoiding harsh reactions in preparing large peptides and proteins. The applicability of this methodology has been demonstrated in the synthesis of an acid-labile therapeutic protein, interferon gamma, and the anticancer activity of synthetic protein has also been evaluated.
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