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Updated: Jan 26, 2026

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids
Published on: August 9, 2024
A rapid derivatization based LC-MS/MS method for quantitation of short chain fatty acids in human plasma and urine
Allan Jaochico1, Dewakar Sangaraju1, Sheerin K Shahidi-Latham1
1Genentech, Drug Metabolism & Pharmacokinetics, 1 DNA Way, South San Francisco, CA 94080, USA.
Abstract:
Aim: Objective of this study is to develop a robust multi-matrix LC-MS/MS for the quantitation of endogenous short-chain fatty acids (SCFA) biomarkers in human plasma and urine. Methods: Developed method utilizes stable isotope-labeled internal standards, high-throughput derivatization procedure for sample preparation and LC-MS/MS analysis using multiple reaction monitoring transitions in positive electrospray ionization mode. Results: Surrogate matrix method was used for quantitation. Accuracy, precision, parallelism, curve linearity, derivatization efficiency, stability and recovery were all evaluated, and the results were well within the acceptable criteria. Conclusion: SCFA levels in human plasma and urine of inflammatory bowel disease patients versus non-disease subjects were quantified and compared by LC-MS/MS.
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