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Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
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Can cytoplasmic donation rescue aged oocytes?
Atsushi Tanaka1, Seiji Watanabe2
1Saint Mother Obstetrics and Gynecology Clinic and Institute for ART Fukuoka Japan.
Reproductive Medicine and Biology
|April 19, 2019
Summary
Assisted reproductive technology (ART) success declines with maternal age due to increased aneuploidy. Spindle chromosome transfer (ST) shows potential for rescuing aged oocytes, offering an alternative to other methods.
Area of Science:
- Reproductive biology
- Genetics
- Assisted reproductive technology
Background:
- Maternal age significantly impacts assisted reproductive technology (ART) outcomes, with decreasing pregnancy and delivery rates.
- Miscarriage rates increase sharply after age 35, exceeding 50% by age 43.
- The incidence of aneuploid fetuses in miscarriages rises with maternal age, surpassing 90% in women over 40.
Purpose of the Study:
- To analyze and compare the efficacy of different cytoplasmic donation technologies for rescuing aged oocytes with high aneuploidy rates.
Main Methods:
- Comparative analysis of various cytoplasmic donation techniques.
- Evaluation of oocyte rescue capabilities, focusing on aneuploidy reduction.
Main Results:
- Germinal vesicle transfer (GVT) is theoretically superior for rescuing meiosis I disjunction but requires further development of in vitro maturation post-GVT.
- Spindle chromosome transfer (ST) demonstrates higher rescue potential, bypassing the need for in vitro maturation.
- ST offers an ethical advantage over pronuclear transfer (PNT) as it avoids embryo destruction.
Conclusions:
- Spindle chromosome transfer (ST) shows promise in rescuing aged oocytes to a certain extent.
- Further basic research is necessary to establish the clinical applicability of cytoplasmic donation for aged oocyte rescue.
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