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Effects of Homeopathic Phosphorus on Encephalitozoon cuniculi-Infected Macrophages In-Vitro

Mirian Yaeko Nagai1, Luciane Costa Dalboni1, Thayná Neves Cardoso1

  • 1Research Center, Graduating Program of Environmental and Experimental Pathology, Universidade Paulista - UNIP, São Paulo, São Paulo, Brazil.

Homeopathy : the Journal of the Faculty of Homeopathy
|April 19, 2019
PubMed
Abstract

Insights

Homeopathic phosphorus (Phos 200cH) modulated infected macrophages, increasing RANTES and lysosome activity. This suggests a potential therapeutic mechanism for Encephalitozoon cuniculi infections.

Area of Science:

  • Immunology
  • Microbiology
  • Homeopathic Medicine

Background:

  • Encephalitozoon cuniculi (E. cuniculi) is an intracellular pathogen causing neurological disease and zoonotic concerns.
  • No established treatment exists, but homeopathic phosphorus showed clinical success via unknown mechanisms.

Purpose of the Study:

  • To investigate if in-vitro infected macrophages change function after homeopathic phosphorus treatment.
  • To analyze parasite internalization, lysosome activity, cytokine production, and cell ultrastructure.

Main Methods:

  • RAW 264.7 macrophages infected with E. cuniculi were treated with varying phosphorus potencies.
  • Analysis included parasite internalization, lysosome activity, cytokine/chemokine production, and cell ultrastructure.

Main Results:

  • Vehicle treatment increased IL-6, TNF-alpha, MCP-1 and reduced parasite internalization.
  • Phos 200cH significantly increased RANTES and lysosome activity over time.
  • Phos 200cH also decreased apoptosis, enhanced parasite digestion, and reduced internalized spores.

Conclusions:

  • Homeopathic phosphorus (Phos 200cH) modulates infected macrophage activity.
  • A key finding is the specific increase in RANTES, crucial for E. cuniculi infection prognosis.

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