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Longitudinal In Vivo Imaging and Quantification of Human Pancreatic Islet Grafting and Contributing Host Cells in the Anterior Eye Chamber
Published on: June 11, 2020
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In Vivo Assessment of Human Islet Potency.
R D Molano1, C Ricordi1, A Pileggi1
1Cell Transplant Center, Diabetes Research Institute, University of Miami, Miami, FL, USA.
Cellr4-- Repair, Replacement, Regeneration, & Reprogramming
|April 20, 2019
Summary
Transplanting insulin-producing cells into mice assesses their function for diabetes research. This in vivo bioassay protocol evaluates cell products for restoring normal blood sugar levels.
Area of Science:
- Endocrinology
- Cell Biology
- Immunology
Background:
- Assessing the viability and function of insulin-producing cells is crucial for diabetes research and clinical applications.
- Current methods require robust bioassays to confirm the efficacy of cellular products aimed at restoring beta cell function.
Purpose of the Study:
- To describe a standardized protocol for the in vivo potency assessment of human islet cell products.
- To detail a bioassay for evaluating the ability of transplanted cells to restore and maintain euglycemia.
Main Methods:
- Utilizing immunodeficient mice as recipients for transplanted insulin-producing cells.
- Inducing chemical diabetes in recipient mice prior to transplantation.
- Monitoring recipients for the restoration and maintenance of euglycemia post-transplantation.
Main Results:
- The described protocol provides a reliable method for assessing the in vivo function of insulin-producing cell products.
- The bioassay successfully evaluates the capacity of transplanted cells to normalize blood glucose levels.
Conclusions:
- The in vivo bioassay is an essential tool for the potency assessment of cellular products for diabetes treatment.
- The protocol is adaptable for various sources of insulin-producing cells, including stem cell-derived and primary cells.
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