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Updated: Jan 26, 2026

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
Published on: October 4, 2017
Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization
Gabriele Pitingolo1, Antoine Riaud2, Claudio Nastruzzi3
1INSERM UMR-S1147, CNRS SNC5014, Paris Descartes University, Equipe Labellisée Ligue Nationale Contre le Cancer, 75005 Paris, France. gabriele.pitingolo@ens.fr.
Abstract:
Traditional two-dimensional (2D) cell culture models are limited in their ability to reproduce human structures and functions. On the contrary, three-dimensional (3D) microtissues have the potential to permit the development of new cell-based assays as advanced in vitro models to test new drugs. Here, we report the use of a dehydrated gelatin film to promote tumor cells aggregation and 3D microtissue formation. The simple and stable gelatin coating represents an alternative to conventional and expensive materials like type I collagen, hyaluronic acid, or matrigel. The gelatin coating is biocompatible with several culture formats including microfluidic chips, as well as standard micro-well plates. It also enables long-term 3D cell culture and in situ monitoring of live/dead assays.
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