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A Comparison of Selected Methods for Determining Sulfite in Prawns
David J Williams1, Steven L Slattery1, Stephen M Nottingham1
1Queensland Food Research Laboratories, Queensland Department of Primary Industries, 19 Hercules Street, Hamilton, Queensland, Australia 4007.
Journal of Food Protection
|April 26, 2019
Summary
Researchers evaluated rapid steam distillation, HPLC, and enzyme tests for sulfite analysis in prawns. HPLC proved most reliable, offering accurate residual sulfite determination comparable to the standard Monier-Williams method.
Area of Science:
- Food Science
- Analytical Chemistry
Background:
- The Monier-Williams distillation is the standard for sulfite analysis but is time-consuming and lacks sensitivity.
- Developing faster, more sensitive methods for residual sulfite determination in food products like prawns is crucial.
Purpose of the Study:
- To assess the suitability of rapid steam distillation, high-performance liquid chromatography (HPLC), and an enzyme-based procedure for routine residual sulfite determination in prawns.
- To compare the accuracy and reliability of these alternative methods against the established Monier-Williams distillation technique.
Main Methods:
- Standard sulfite solutions and prawns (known and unknown levels) were analyzed using rapid steam distillation, HPLC, and an enzyme test.
- Results were statistically compared with those obtained from the Monier-Williams distillation.
Main Results:
- Rapid steam distillation showed close agreement with Monier-Williams for standard solutions but required a correction factor for prawn analysis.
- High-performance liquid chromatography (HPLC) provided results in close agreement with the Monier-Williams method for both standard solutions and prawns.
- The enzyme test was unsatisfactory for sulfite analysis in prawns, despite initial promise with standard solutions.
Conclusions:
- High-performance liquid chromatography (HPLC) is a suitable and reliable alternative for routine residual sulfite determination in prawns.
- Rapid steam distillation may be viable with appropriate calibration, while the enzyme test is not recommended for this application.
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