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Converting Any Faradaic Current Generated at an Electrode under Potentiostatic Control into a Remote Fluorescence
Rabia Djoumer1, Agnès Anne1, Arnaud Chovin1
1Laboratoire d'Electrochimie Moléculaire, UMR 7591 CNRS , Université Paris Diderot , Sorbonne Paris Cité, 15 rue Jean-Antoine de Baïf , Paris F-75205 Cedex 13 , France.
Analytical Chemistry
|April 30, 2019
Summary
We developed a new method to convert electrochemical signals into fluorescence. This technique uses a fluorescent reporter molecule to track electrochemical reactions, enabling sensitive detection of nonfluorescent species.
Area of Science:
- Electrochemistry
- Analytical Chemistry
- Fluorescence Spectroscopy
Background:
- Electrochemical methods offer sensitive detection but often lack visual output.
- Fluorescence spectroscopy provides high sensitivity and visual detection but can be complex to couple with electrochemistry.
Purpose of the Study:
- To develop an original faradaic current-to-fluorescence conversion scheme.
- To couple electrochemical reactions with fluorescence emission for quantitative signal transduction.
- To validate the scheme using resazurin as a fluorescent reporter molecule.
Main Methods:
- Simultaneous recording of cyclic voltammograms and voltfluorograms.
- Studying the reduction mechanism of resazurin.
- Utilizing numerical simulations to analyze electrochemical and fluorescence signals.
- Implementing a potentiostatic setup with a micrometer-sized electrode at the counter electrode.
Main Results:
- Detailed kinetic study of resazurin reduction.
- Demonstrated perfect tracking of faradaic current by fluorescence emission at a micrometer-sized electrode.
- Quantitative transduction of nonfluorescent species oxidation into simultaneous fluorescence emission.
Conclusions:
- The developed faradaic current-to-fluorescence conversion scheme is effective for sensitive detection.
- This method allows for the quantitative conversion of electrochemical signals into fluorescence.
- The strategy holds promise for various analytical applications requiring sensitive and visual detection of electrochemical reactions.
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