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Related Experiment Video

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Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
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Advances in aptamer screening technologies.

Jianhua Yan1, Hongjie Xiong1, Shundong Cai1

  • 1Department of Pharmaceutics, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, Hunan Province, PR China.

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|May 1, 2019
PubMed
Summary
This summary is machine-generated.

Systematic evolution of ligands by exponential enrichment (SELEX) screens aptamers for bio-sensing and therapeutics. This review covers SELEX technologies, their applications, and future directions for efficient aptamer discovery.

Keywords:
AptamerOligonucleotide librarySELEXScreening

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Systematic evolution of ligands by exponential enrichment (SELEX) is a key technology for aptamer discovery.
  • Aptamers are increasingly used in bio-sensing and targeted therapeutics.
  • SELEX technology has undergone significant development and diversification.

Purpose of the Study:

  • To review current SELEX screening technologies.
  • To analyze the principles, advantages, and disadvantages of various SELEX methods.
  • To summarize aptamers developed via SELEX and their clinical relevance.

Main Methods:

  • Review of established and novel SELEX techniques including cell-SELEX, mag-SELEX, and capillary electrophoresis SELEX.
  • Analysis of screening principles, efficiency, and specificity.
  • Compilation of aptamers identified through SELEX and their applications.

Main Results:

  • Various SELEX modifications offer diverse approaches to aptamer screening.
  • Aptamers screened by SELEX show promise in diagnostics and therapeutics.
  • Challenges remain in achieving high-affinity aptamers efficiently.

Conclusions:

  • SELEX technology is crucial for aptamer development in various fields.
  • Continued innovation in SELEX methods is needed to overcome current limitations.
  • Future research should focus on enhancing aptamer affinity and screening efficiency.