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Terahertz Microfluidic Sensing Using a Parallel-plate Waveguide Sensor
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Liquid-core waveguide TCSPC sensor for high-accuracy fluorescence lifetime analysis.

Liping Wei1, Yi Tian1, Wenrong Yan1

  • 1Department of Materials Science and Engineering, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong.

Analytical and Bioanalytical Chemistry
|May 1, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces a compact liquid-core waveguide time-correlated single-photon counting sensor for fluorescence lifetime measurement. The optimized sensor minimizes temporal dispersion, enabling accurate measurements for microfluidic and point-of-care applications.

Keywords:
Excitation rejectionFluorescence lifetimeLiquid-core waveguideMultipath propagationTCSPCTemporal dispersion

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Area of Science:

  • Photonics and Spectroscopy
  • Microfluidics
  • Analytical Chemistry

Background:

  • Liquid-core waveguides (LCWs) offer artifact-free optical detection but are limited in fluorescence lifetime measurement (FLM) due to temporal dispersion.
  • Traditional FLM systems often rely on lenses and filters, which can introduce optical artifacts.
  • Steady-state fluorescence detection is common in microsystems, but FLM requires specialized approaches for LCWs.

Purpose of the Study:

  • To develop a compact liquid-core waveguide time-correlated single-photon counting (LCW-TCSPC) sensor for accurate fluorescence lifetime measurement (FLM).
  • To analyze and optimize the propagation of excitation light within the LCW for enhanced FLM performance.
  • To demonstrate the feasibility of LCW-based FLM for microfluidic and point-of-care diagnostics.

Main Methods:

  • Analytical and simulation-based analysis of excitation propagation within the LCW.
  • Experimental characterization of the LCW-TCSPC sensor prototype.
  • Optimization of excitation propagation length to minimize temporal dispersion and maximize excitation rejection.

Main Results:

  • Analytical and simulation results for excitation propagation in LCW were validated by experimental characterization.
  • An optimal region within the LCW was identified for highly accurate FLM.
  • The prototype demonstrated excellent excitation rejection and low temporal dispersion.
  • A detection limit of 5 nM for Coumarin 6 in DMSO was achieved with <3% lifetime error.

Conclusions:

  • The developed LCW-TCSPC sensor effectively enables accurate FLM, overcoming limitations of temporal dispersion.
  • Optimization of excitation propagation within the LCW is crucial for high-performance FLM.
  • The proposed techniques and prototype pave the way for advanced microfluidic and point-of-care FLM applications.