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Identification of biomarkers in macrophages of atherosclerosis by microarray analysis

He-Ming Huang1, Xin Jiang1, Meng-Lei Hao2

  • 1Geriatric Department, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen, 518020, People's Republic of China.

Lipids in Health and Disease
|May 3, 2019
PubMed

Insights

This study identified key genes in macrophages involved in atherosclerosis (AS). These differentially expressed genes and hub genes, like KDELR3, CD55, and DYNC2H1, may serve as novel biomarkers for atherosclerotic cardiovascular disease (ASCVD).

Area of Science:

  • Biomedical research
  • Cardiovascular science
  • Genomics

Background:

  • Atherosclerotic cardiovascular disease (ASCVD) is a major global cause of death.
  • Macrophages are critical in atherosclerosis (AS) development, but their specific targets remain unclear.
  • This study focuses on identifying molecular targets within AS macrophages.

Purpose of the Study:

  • To screen and identify molecular targets in AS macrophages using bioinformatics.
  • To understand the role of macrophages in AS pathogenesis.

Main Methods:

  • Utilized Gene Expression Omnibus datasets (GSE7074, GSE9874) to identify differentially expressed genes (DEGs) in AS macrophages.
  • Performed functional annotation using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases.
  • Constructed a protein-protein interaction network and identified hub genes using STRING and Cytoscape.

Main Results:

  • Identified 98 DEGs between non-AS and AS macrophages.
  • Enriched functions included response to hypoxia, respiratory gaseous exchange, and protein binding.
  • Discovered three hub genes: KDELR3, CD55, and DYNC2H1.

Conclusions:

  • The identified DEGs and hub genes show potential as diagnostic and therapeutic biomarkers for AS.
  • These findings contribute to understanding macrophage-driven AS.
  • Further validation of KDELR3, CD55, and DYNC2H1 in AS is warranted.
Abstract

Keywords:
AtherosclerosisAtherosclerotic cardiovascular diseaseBioinformaticsHub genesMacrophages

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