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Updated: Jan 25, 2026

Directly Measuring Forces Within Reconstituted Active Microtubule Bundles
Published on: May 10, 2022
DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis
Miquel Rosas-Salvans1, Jacopo Scrofani1, Aitor Modol1
1Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology, Dr Aiguader 88, 08003 Barcelona, Spain.
Abstract:
Bipolar spindle organization is essential for the faithful segregation of chromosomes during cell division. This organization relies on the collective activities of motor proteins. The minus-end-directed dynein motor complex generates spindle inward forces and plays a major role in spindle pole focusing. The dynactin complex regulates many dynein functions, increasing its processivity and force production. Here, we show that DnaJB6 is a novel RanGTP-regulated protein. It interacts with the dynactin subunit p150Glued (also known as DCTN1) in a RanGTP-dependent manner specifically in M-phase, and promotes spindle pole focusing and dynein force generation. Our data suggest a novel mechanism by which RanGTP regulates dynein activity during M-phase.
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