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Updated: Jan 25, 2026
07:44
In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
Published on: July 24, 2020
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Scanning laser optical tomography in a neuropathic mouse model : Visualization of structural changes.
J Schulze1,2, L Nolte3,4, S Lyutenski5
1Department of Otorhinolaryngology, Head and Neck Surgery, Lower Saxony Center for Biomedical Engineering, Implant Research and Development (NIFE), Hannover Medical School, Stadtfelddamm 34, 30625, Hannover, Germany. Schulze.Jennifer.HNO@mh-hannover.de.
HNO
|May 9, 2019
Summary
Scanning laser optical tomography (SLOT) visualizes whole mouse cochleae, detecting structural changes like reduced nerve fibers in Cav1.3-/- models. This method is suitable for gross changes but not fine cochlear structures.
Area of Science:
- Hearing research
- Otolaryngology
- Biomedical imaging
Background:
- Traditional cochlear imaging requires destructive sample preparation.
- Scanning Laser Optical Tomography (SLOT) offers non-destructive visualization of the entire cochlea.
- Cav1.3-/- mice exhibit known inner ear structural alterations, serving as a model.
Purpose of the Study:
- To evaluate SLOT's efficacy in detecting structural differences in the murine cochlea.
- To assess SLOT's capability in identifying phenotypic changes in a mouse model of hearing impairment.
Main Methods:
- Whole, intact cochleae from Cav1.3-/- and wild-type mice were analyzed using SLOT.
- Immunostaining and osmium tetroxide staining were employed.
- Results were compared with fluorescence microscopy analyses.
Main Results:
- SLOT revealed an absence of nerve fiber staining in Cav1.3-/- mice at P27 compared to wild-type.
- Confirmation of reduced radial nerve fibers and ribbon synapses per inner hair cell in the cochlear apex.
- Significant reduction in ribbon synapses observed at P20 and P27 in the apical cochlea of mutant mice.
Conclusions:
- SLOT is effective for analyzing gross structural changes in non-dissected cochleae.
- The Cav1.3-/- mouse model demonstrated SLOT's utility in identifying major phenotypic alterations.
- For detailed fine structural analysis, complementary methods remain necessary.
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