Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

932
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
932
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

8.5K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
8.5K
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

7.0K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
7.0K
Magnetic Susceptibility and Permeability01:31

Magnetic Susceptibility and Permeability

2.3K
In linear magnetic materials, like paramagnets and diamagnets, magnetization is proportional to the magnetic field intensity. The constant of proportionality, a dimensionless number, is called magnetic susceptibility. The value of the susceptibility depends on the type of material.
When diamagnetic materials are placed under an external magnetic field, the moments opposite to the field are induced. Hence, the susceptibility for diamagnets has a minimal negative value of 10-5–10-6. Since...
2.3K
Antimicrobial Proteins01:23

Antimicrobial Proteins

13.1K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
13.1K
Classification of Neurotransmitters01:30

Classification of Neurotransmitters

5.2K
Neurotransmitters play a crucial role in the communication between neurons in the autonomic nervous system. Neurons in the autonomic nervous system can be cholinergic or adrenergic depending on the neurotransmitters synthesized. Cholinergic neurons use acetylcholine as their primary neurotransmitter. This includes all the preganglionic fibers of the sympathetic and pre- and postganglionic fibers of the parasympathetic nervous systems. In addition, neurons of the somatic nervous system also use...
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integration of multiomic and multi-phenotypic data identifies biological pathways associated with physical fitness.

Communications biology·2026
Same author

A neural network model delivers a highly prognostic protein signature in cancer stem cells that identifies relapse in stage III colorectal cancer patients.

bioRxiv : the preprint server for biology·2026
Same author

Transient Transfection of Rolling-Circle Amplified DNA in Biomanufacturing-Relevant Mammalian Cell Lines: A Comparison of Transfection Conditions for Optimal Protein Expression.

Biotechnology and applied biochemistry·2025
Same author

Sample preparation using multiple microbial pattern recognition proteins and magnetic bead ratcheting.

SLAS technology·2025
Same author

Integrated multiplex analysis of cell death regulators in stage II colorectal cancer suggests patients with 'persister' cell profiles fail to benefit from adjuvant chemotherapy.

BMJ oncology·2025
Same author

Transspinal Focused Ultrasound Suppresses Spinal Reflexes in Healthy Rats.

Neuromodulation : journal of the International Neuromodulation Society·2023
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Related Experiment Video

Updated: Jan 25, 2026

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

3.3K

Adaptable microfluidic system for single-cell pathogen classification and antimicrobial susceptibility testing.

Hui Li1, Peter Torab2, Kathleen E Mach3

  • 1Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802.

Proceedings of the National Academy of Sciences of the United States of America
|May 10, 2019
PubMed
Summary
This summary is machine-generated.

A new microfluidic system rapidly classifies bacterial pathogens and determines antimicrobial susceptibility (AST) at the single-cell level within 30 minutes. This innovation aids prompt infection treatment and antibiotic stewardship.

Keywords:
antimicrobial susceptibility testingdiagnosticsinfectionmicrofluidicssingle-cell analysis

More Related Videos

Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format
12:39

Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format

Published on: June 24, 2011

38.8K
Stress-induced Antibiotic Susceptibility Testing on a Chip
12:41

Stress-induced Antibiotic Susceptibility Testing on a Chip

Published on: January 8, 2014

6.9K

Related Experiment Videos

Last Updated: Jan 25, 2026

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

3.3K
Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format
12:39

Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format

Published on: June 24, 2011

38.8K
Stress-induced Antibiotic Susceptibility Testing on a Chip
12:41

Stress-induced Antibiotic Susceptibility Testing on a Chip

Published on: January 8, 2014

6.9K

Area of Science:

  • Microbiology
  • Biotechnology
  • Medical Diagnostics

Background:

  • Bacterial infections cause significant global morbidity and mortality.
  • Rapid diagnostics are crucial for effective treatment and antibiotic stewardship.
  • Current antimicrobial susceptibility testing (AST) methods are time-consuming.

Purpose of the Study:

  • To develop and validate an adaptable microfluidic system for rapid pathogen classification and AST.
  • To enable single-cell level analysis for precise bacterial identification and susceptibility determination.
  • To reduce the time required for microbiological analysis from days to minutes.

Main Methods:

  • Utilized a microfluidic system with tunable valves and real-time optical detection.
  • Trapped bacteria for classification based on physical characteristics (shape, size).
  • Monitored bacterial growth in the presence of antibiotics at the single-cell level for AST.

Main Results:

  • The microfluidic system achieved 100% sensitivity and 83.33% specificity for pathogen classification.
  • Demonstrated 100% concordance for antimicrobial susceptibility testing (AST).
  • Successfully detected pathogens in various sample types, including urine, blood cultures, and whole blood, including polymicrobial samples.

Conclusions:

  • The microfluidic platform offers rapid, single-cell level pathogen classification and AST.
  • This technology significantly shortens diagnostic timelines compared to standard methods.
  • The system shows high potential for clinical application in diagnosing and managing bacterial infections.