Related Experiment Video
Updated: Jan 25, 2026
10:59
Analysis of SEC-SAXS data via EFA deconvolution and Scatter
Published on: January 28, 2021
9.8K
Re-certification of hydroxyvitamin D standards by isotope pattern deconvolution
J Pitarch-Motellón1, A F Roig-Navarro1, C Le Goff2
1Research Institute for Pesticides and Water, Universitat Jaume I, Castelló, Spain.
Summary
Accurate vitamin D metabolite measurements are crucial. Isotope pattern deconvolution (IPD) using LC-MS/MS corrects commercial standards, ensuring reliable vitamin D testing and meeting regulatory requirements.
Area of Science:
- Clinical Chemistry
- Analytical Chemistry
- Mass Spectrometry
Background:
- Vitamin D testing demand is rising due to its role in various health disorders.
- Accurate quantification of vitamin D metabolites in human serum is essential due to narrow medical significance ranges.
- Commercial standards require rigorous validation for reliable vitamin D analysis.
Purpose of the Study:
- To present an isotope dilution mass spectrometry (IDMS) method for re-certifying commercial vitamin D standards.
- To validate the use of isotope pattern deconvolution (IPD) for standard re-certification.
- To ensure accuracy and precision in vitamin D metabolite measurements.
Main Methods:
- Isotope dilution mass spectrometry (IDMS) quantification.
- Isotope pattern deconvolution (IPD) technique.
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.
Main Results:
- IPD effectively compensated for biases in standard concentrations: +4.7% for 25(OH)D3, -29% for 25(OH)D2, and -30% for 24,25(OH)2D3.
- The IPD method offers an easy, cost-effective, and straightforward approach to standard correction.
- Identified discrepancies between stated and actual values of commercial standards, highlighting the need for re-certification.
Conclusions:
- Discrepancies in commercial standards can introduce bias into analytical methodologies.
- The IPD correction method ensures compliance with international standardization programs like the Vitamin D Standardization Program (VDSP).
- Accurate standard re-certification is vital for the reliability of clinical vitamin D testing.
Related Concept Videos
01:12Isotopes
63.5K
Elements have a set number of protons that determines their atomic number (Z). For example, all atoms with eight protons are oxygen; however, the number of neutrons can vary for atoms of the same element. The sum of the number of protons and the number of neutrons is the mass number (A). Atoms with the same atomic number but different mass numbers are called isotopes. Elements can have multiple isotopes, for example, carbon-12, carbon-13, and carbon-14.
An element's atomic mass, or weight,...
An element's atomic mass, or weight,...
63.5K
01:20Deconvolution
562
Deconvolution, also known as inverse filtering, is the process of extracting the impulse response from known input and output signals. This technique is vital in scenarios where the system's characteristics are unknown, and they must be inferred from the observable signals.
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...
562
02:31Elements: Chemical Symbols and Isotopes
125.5K
A chemical symbol is an abbreviation used to indicate an element or an atom of an element. For example, the symbol for mercury is Hg. The same symbol is used to indicate one atom of mercury (microscopic domain) or to label a container of many atoms of the element mercury (macroscopic domain).
Some symbols are derived from the common English name of the element; others are abbreviations of the name in another language — Latin, Greek or German. For example, the symbol for aluminum (common name)...
Some symbols are derived from the common English name of the element; others are abbreviations of the name in another language — Latin, Greek or German. For example, the symbol for aluminum (common name)...
125.5K
01:28Isotopes and Radioisotopes
11.3K
In the early 1900s, English chemist Frederick Soddy realized that an element could have atoms with different masses that were chemically indistinguishable. These different types are called isotopes — atoms of the same element that differ in mass. Isotopes differ in mass because they have different numbers of neutrons but are chemically identical because they have the same number of protons. Soddy was awarded the Nobel Prize in Chemistry in 1921 for this discovery.
An isotope containing...
An isotope containing...
11.3K
01:13Mass Spectrometry: Isotope Effect
4.0K
Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
4.0K
01:06Fixed Action Patterns
17.6K
A fixed action pattern (FAP) is a specific, hard-wired sequence of behaviors that occurs in response to an external stimulus, called a sign stimulus. The behavior is “fixed” because it is essentially unchangeable—proceeding similarly across individuals of a species every time it occurs.
17.6K