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Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
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Draft Genome Sequence of a Rare Israeli Clinical Isolate of Burkholderia pseudomallei

Ofir Israeli1, Inbar Cohen-Gihon1, Tal Brosh-Nissimov2,3

  • 1Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.

Microbiology Resource Announcements
|May 11, 2019
PubMed

Insights

We sequenced the genome of Burkholderia pseudomallei MAA2018, a virulent bacterium. This strain caused the first reported melioidosis case in Israel, linked to travel in India.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Melioidosis is a serious infectious disease caused by Burkholderia pseudomallei.
  • The bacterium is endemic in Southeast Asia and Australia.
  • Recent cases suggest a potential expansion of its geographical range.

Purpose of the Study:

  • To perform whole-genome sequencing of the Burkholderia pseudomallei MAA2018 strain.
  • To analyze the genetic makeup of a strain responsible for a novel melioidosis case in Israel.
  • To provide genomic data for understanding B. pseudomallei epidemiology and evolution.

Main Methods:

  • Whole-genome sequencing of B. pseudomallei MAA2018.
  • Bioinformatic analysis of the draft genome sequence.

Main Results:

  • The draft genome sequence of Burkholderia pseudomallei MAA2018 was successfully generated.
  • The strain was isolated from the first Israeli melioidosis patient with a travel history to Goa, India.

Conclusions:

  • The availability of the B. pseudomallei MAA2018 genome sequence aids in understanding the bacterium's genetic diversity.
  • This genomic data can contribute to epidemiological surveillance and the development of diagnostic and therapeutic strategies for melioidosis.

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