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Updated: Jan 25, 2026
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How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays MEAs
Published on: May 30, 2010
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Multisite Intracellular Recordings by MEA.
Micha E Spira1,2,3, Shun-Ho Huang4,5,6, Nava Shmoel4,5,6
1Department of Neurobiology, The Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem, Jerusalem, Israel. Spira@cc.huji.ac.il.
Advances in Neurobiology
|May 11, 2019
Summary
New 3D vertical nanoelectrode arrays enable intracellular recordings, capturing full electrophysiological signals from single cells and networks. This technology advances brain research and personalized medicine by overcoming limitations of traditional extracellular recordings.
Area of Science:
- Neuroscience and Bioengineering
- Materials Science and Nanoelectronics
Background:
- Traditional planar multielectrode arrays (MEAs) are limited to extracellular field potentials, missing crucial intracellular electrophysiological signaling.
- Advances in materials science and nanoelectronics enable the development of new MEA technologies for intracellular recordings.
Purpose of the Study:
- To present the achievements of 3D vertical nanoelectrode MEA technology for intracellular recordings.
- To explain the principles, configurations, and recorded signal characteristics of this novel MEA technology.
- To discuss challenges and solutions for long-term intracellular access.
Main Methods:
- Development of 3D vertical nanoelectrodes designed to penetrate cell membranes for intracellular access.
- Utilizing nanochip/microchip fabrication for scaling up to hundreds of simultaneous recordings.
- Functional integration of nanoelectrodes with cultured neurons, cardiomyocytes, and myotubes.
Main Results:
- Successful recording of attenuated intracellular action potentials and synaptic potentials from individual cells.
- Demonstration of simultaneous intracellular recordings from hundreds of cultured cardiomyocytes.
- Characterization of the quality and features of intracellular signals obtained by vertical nanoelectrodes.
Conclusions:
- 3D vertical nanoelectrode MEA technology offers a significant advancement over planar MEAs for comprehensive electrophysiological recordings.
- This technology holds great promise for in vitro drug screening, personalized medicine, and fundamental brain research.
- Overcoming current limitations in intracellular access duration is key for future applications.
Keywords:
Action potentialsCardiomyocytesElectroporationIntracellular recordingsMembrane repairMushroom-shaped microelectrodesNeuronsOptoporationSeal resistanceStriated myotubesSynaptic potentialsVertical nanoelectrodesMore Related Videos
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