Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

20.1K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
20.1K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

11.1K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.1K
Reflection of Waves01:07

Reflection of Waves

4.5K
When a wave travels from one medium to another, it gets reflected at the boundary of the second medium. A common example of this is when a person yells at a distance from a cliff and hears the echo of their voice. The sound waves (longitudinal waves) traveling in the air are reflected from the bounding cliff. Similarly, flipping one end of a string whose other end is tied to a wall causes a pulse (transverse wave) to travel through the string, which gets reflected upon reaching the wall. In...
4.5K
The Sense of Self: Reflected Self-Appraisal and Social Comparison02:57

The Sense of Self: Reflected Self-Appraisal and Social Comparison

55.6K
According to Charles Cooley, we base our image on what we think other people see (Cooley 1902). We imagine how we must appear to others, then react to this speculation. We don certain clothes, prepare our hair in a particular manner, wear makeup, use cologne, and the like—all with the notion that our presentation of ourselves is going to affect how others perceive us. We expect a certain reaction, and, if lucky, we get the one we desire and feel good about it. But more than that, Cooley...
55.6K
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

1.2K
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
1.2K
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

13.0K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
13.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An anti-PMEL antibody-drug conjugate with a G<sub>q/1</sub><sub>1</sub> inhibitor payload in GNAQ/GNA11-mutant melanomas: a phase 1 trial.

Nature medicine·2026
Same author

Robustness of a neural network approved for skin cancer diagnosis against variations in sequential images.

Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·2026
Same author

[Multiple papules on the thighs in an adult].

Dermatologie (Heidelberg, Germany)·2026
Same author

Hierarchical classification of immune cell transcriptomes at population-scale.

bioRxiv : the preprint server for biology·2026
Same author

Multiclass convolutional neural network vs. 96 dermatologists in skin lesion diagnosis, an international study.

Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·2026
Same author

The Endocytic Receptor DEC205 Is Expressed by Brain Endothelial Cells and Is Involved in Regulating the Blood-Brain Barrier.

Cells·2026

Related Experiment Video

Updated: Jan 25, 2026

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy
10:22

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy

Published on: November 29, 2017

9.6K

[Reflectance confocal microscopy in Shiitake dermatitis].

Christiane S Cussigh1, Christine Fink1, Julia K Winkler1

  • 1Klinik für Dermatologie, Venerologie und Allergologie, Universitätsklinikum Heidelberg (UKH), Ruprecht-Karls-Universität, Im Neuenheimer Feld 440, 69120, Heidelberg, Deutschland.

Der Hautarzt; Zeitschrift Fur Dermatologie, Venerologie, Und Verwandte Gebiete
|May 16, 2019
PubMed
Summary

Shiitake dermatitis, a rash from raw shiitake mushrooms, is caused by lentinan. Reflectance confocal microscopy (RCM) shows promise for diagnosing this condition non-invasively.

Keywords:
Diagnostic imagingEczemaLentinanPruritusShiitake mushroom

More Related Videos

Preparing Porcine Eyes for Confocal Reflectance Microscopy to Visualize the Vitreous Collagen Fiber Network
06:07

Preparing Porcine Eyes for Confocal Reflectance Microscopy to Visualize the Vitreous Collagen Fiber Network

Published on: October 17, 2025

354
Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

28.6K

Related Experiment Videos

Last Updated: Jan 25, 2026

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy
10:22

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy

Published on: November 29, 2017

9.6K
Preparing Porcine Eyes for Confocal Reflectance Microscopy to Visualize the Vitreous Collagen Fiber Network
06:07

Preparing Porcine Eyes for Confocal Reflectance Microscopy to Visualize the Vitreous Collagen Fiber Network

Published on: October 17, 2025

354
Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

28.6K

Area of Science:

  • Dermatology
  • Mycology
  • Medical Imaging

Background:

  • Shiitake dermatitis is an inflammatory skin reaction.
  • It typically appears 1-2 days after consuming raw or undercooked shiitake mushrooms.
  • The condition presents as linear, itchy, red papulovesicular rashes.

Observation:

  • Lentinan, a polysaccharide from shiitake mushroom cell walls, is identified as the causative agent.
  • Histological examination reveals eczema-like features, including spongiosis and lymphohistiocytic infiltrates.
  • Reflectance confocal microscopy (RCM) findings align with histopathological results.

Findings:

  • Reflectance confocal microscopy (RCM) demonstrates a correlation with traditional histopathological findings in shiitake dermatitis.
  • This suggests RCM can effectively visualize the characteristic microscopic changes of the condition.

Implications:

  • Reflectance confocal microscopy (RCM) offers a potential non-invasive method for diagnosing shiitake dermatitis.
  • This could lead to faster and more accessible diagnostic confirmation for patients.
  • Further research may establish RCM as a standard tool in clinical dermatology for this mushroom-induced reaction.